Electrophoresis

Electrophoresis is an analytical method in which a controlled electric current is used to separate biomolecules according to their size to the electric charge ratio, using a gelatinous matrix as a base.

This technique has a variety of practical uses, such as forensic medicine for human identification, the human genome project, genetic and protein mutation research, and clinical diagnostic testing.

What equipment is used in this technique?

Electrophoresis is performed with equipment made up of a negative charge at one end and a positive charge at the other, called an electrophoresis system. When inserting charged molecules, in this environment, the negative ones will go to one extreme and the positive ones to the other corresponding.

For example, when analyzing proteins on a gel, in these kits, the entire protein is taken to analyze its size. In this way, the shorter ones will migrate to the poles more quickly and will be reflected in the lower part of the gel. Instead, the longest will be at the top.

What is electrophoresis?

Electrophoresis is an analytical method in which a controlled electric current is used in order to separate biomolecules according to their size-to-electric charge ratio, using a gelatinous matrix as a base. This technique has a variety of practical uses, such as forensic medicine for the identification of people, the human genome project, protein and genetic mutation research, and clinical diagnostic tests.

What equipment is used in this technique?

Electrophoresis is carried out with equipment composed of a negative charge at one end and a positive charge at the other, called an electrophoresis system. When inserting charged molecules, in this environment, the negative ones will go to one extreme and the positive ones to the other corresponding.

Our best-selling Electrophoresis System:

Vertical Mini-Protean Electrophoresis Cell YR03426

With two gel casts, you can cast 1 to 4 plates of gels at the same time. There are two methods of "casting gel in original position" and "multi casting gel" as an option.

Installing the glass plate in just 15 seconds, and you don't need the button, it's quick and convenient.
With a gel mold in its original position, it is not necessary to move the vessels from gel fabrication to electrophoresis. It is more convenient to observe the gel preparation on both sides of the vessels.
High transparency polycarbonate material injection molding, impact resistance, high temperature resistance, corrosion resistance.

At Kalstein you can find the ideal electrophoresis system for your laboratory.

Because there are multiple laboratory electrophoresis systems, it is important to consider the following steps when using these instruments:

Agarose gel preparation

Weigh the amount of agarose necessary to obtain the desired concentration as a function of the gel volume. Add the agarose to the buffer. Heat the mixture in a microwave oven until it has melted

Sample preparation

Mix both DNA samples and size marker with 0.2 volumes of 6x loading buffer. The total volume will be determined by the size of the wells, usually 15-30 Β΅l.

Loading the samples and running the gel

Once the gel has solidified, remove the seal from the edges and place the mold with the gel in the electrophoresis chamber. Add electrophoresis buffer (TAE 1x or TBE 0.5x) until it covers the.

Gel staining and DNA visualization

If Ethidium Bromide was not added to the gel, the gel should be stained after electrophoresis is complete. To do this, the gel is removed from its mold and immersed in a solution of ethidium bromide (0.5 Β΅g / ml).

Types of Electrophoresis Systems:

Vertical Electrophoresis

Vertical gel electrophoresis is a more complex setup compared to horizontal gel system. Researchers typically use this system to separate proteins rather than nucleic acids. However, before separating the proteins, you must break the quaternary structure of the proteins.

Horizontal Electrophoresis

Cellulose acetate membrane electrophoresis (CAME) is one of the more classical approaches to protein panel analysis. In clinical practice, urinary protein electrophoresis has been used primarily to confirm Bence Jones protein in multiple myeloma 6, but it has not been widely used.

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What is the ideal laboratory electrophoresis?

There are countless models, so it is normal that you do not know which laboratory electrophoresis system to buy to suit your needs. At Kalstein, we evaluate them to find what you're looking for.

Electrophoresis: steps for a run of gels

Electrophoresis is a molecular biology technique where controlled electric current is used to separate biomolecules according to their size to electric charge ratio, using a gelatinous matrix (gel) as a base.

What is the electrophoresis method?

Electrophoresis is an analytical method in which a controlled electric current is used in order to separate biomolecules according to their size-to-electric charge ratio, using a gelatinous matrix as a base ...

Horizontal and vertical electrophoresis

Gel electrophoresis is a laboratory technique used in genetics to separate mixtures containing DNA, RNA and other proteins according to their respective molecular size and charge. DNA, RNA or proteins that must ...

Electrophoresis and Gel Documentation Systems

Gel electrophoresis is a widely used technique in life science laboratories to separate macromolecules such as DNA, RNA, and proteins. In this technique, molecules are separated according to size and electrical charge.

How does electrophoresis work?

The gel used in gel electrophoresis is generally made from a material called agarose, which is a gelatinous substance extracted from seaweed. This porous gel could be used to separate macromolecules of many different sizes.

The Electrophoresis System:

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