The microplate reader is a laboratory equipment developed for the quantification of proteins and nucleic acids through cell images. It is based on filtration of proteins deposited in wells of a plastic plate adjusted to aid in absorption and bind antibodies.
In this case, the microplate reader is one of the methods applied to diagnose cancer, which begins with the blood sample and is based on the determined interaction between antigens and antibodies, where the discovery is executed by substances and enzymatic reactions.
And Kalstein has the ideal equipment, because thanks to its functions he performs the studies with the Elisa technique, where the microplate reader uses the antibodies and measures the amount of the substance in serum where an enzymatic reaction occurs that changes color and records the results. This equipment is especially used to detect viral diseases. Thanks to fluorescence measurements, it detects absorbance, luminescence in a specialized way such as time-resolved fluorescence (TRF) and fluorescence polarization (PF).
Microplate Reader Efficiency
Currently, it has been shown that in the majority of the female population the worldwide incidence of ovarian cancer is the most common cause of mortality after breast, colorectal, lung and uterine cancer, due to many factors such as age, menopause, diet, overweight, physical activity, continuing pregnancies, drugs, smoking, alcohol and family history. And thanks to the early diagnosis by the Elisa method applied by the microplate reader, it provides the necessary and timely information for the monitoring of the disease.
Its functions are based on separating the immune complex created on the solid base and unfixed particles, which is performed by aspiration and washing, facilitating the removal of waste that may interfere with readings. The separation is done by sedimentation where the immune complexes of interest are taken, filtration, radial diffusion and other immunochromatographic principles that work for quick and simple practices.
In detail, the microplate reader offered by Kalstein allows the readings of the results to be reliable thanks to the external jet printer, shows the reports of the multiformat studies, because it also has the appropriate technology and an 8-channel optical system, which allows testing in a quantitative and qualitative way, and are ideal to apply the different methods, both direct, indirect, secondary and sandwich type being the most recommended method for the automation of the technique of Elisa.
Determination of Tumor Markers. Elisa technique
Using the microplate reader and the Elisa technique, it will allow to determine tumor markers through the following steps of extraction and analysis, which are:
- The sample should be removed by vein and placed in a tube without any additive or coagulant content.
- The blood must clot.
- Centrifuge the samples at 1200 rpm for 10mn.
- Using a pipette, transfer the centrifuged serum into prepared or coded tubes.
- Separate the serum samples into a stand.
- Store samples in refrigerators at room temperature.
- Have the washing solutions ready before use, diluting distilled water with amounts of 1000 mL, then calibrate and obtain the check curve.
- Place samples in the microplate wells.
- Incubate for 1 hour at room temperature.
- Remove the reaction mixture
- Incubate for 15 min at room temperature protected from light.
- Place the sample in the microplate wells
- Shake the microplate very carefully
- Finally, read the absorbance at 450 nm versus white within 5 mn.
In this way, the frequency of risk factors and tumor marker are obtained.
Kalstein Brand Microplate Reader
At Kalstein we are manufacturers and pioneers in technological applications designed for our Microplate Readers, which we offer to our customers. They belong to the YR series, it has a reading range of 0.000-4.000 Abs. 405nm, 492nm, 630nm wavelength. They provide connectivity and intelligence.
To learn more about our team, we invite you to take a look at our catalog from HERE