Detecting COVID-19 using the Microplate Reader

The microplate reader is a laboratory equipment that provides resistance, sensitivity and wellbeing for a wide variety of practices, using the Elisa technique to eliminate bacterial proliferation and protein quantification. It has an 8-channel head allowing fast readings, thanks to the technology it has to measure and control samples deeply.

At the same time, the use of the microplate reader to diagnose the respiratory problems caused by covid-19 becomes the ideal tool because it allows measuring the rate of actual infection and the number of infected by the presence of certain antiviral antibodies, also allows identifying asymptomatic infections.

Therefore, Kalstein offers the ideal microplate reader that combines a wide application base and appropriate technology for validation and adaptation for all laboratories in the market.

COVID-19 Microplate Reader Performance

The acute respiratory syndrome caused by covid-19, is a virus detected in December 2019 which expanded worldwide causing multiple complications by the level of human-to-human transmission, with very rapid incubation periods. For the diagnosis, methods of studies in plasma sample were used to demonstrate if the immune system is in contact with the virus.

In addition, virus detection tests are used to monitor and control outbreaks, and using the microplate reader measurement is performed on the 96-well plate, where the values of the practice calibrators are defined by the solution of a highly purified covid-19 IgG antidote in a matrix. The calibrator and positive and negative records meet the criteria for batch analysis, showing efficient results.

For this reason, with the use of the microplate reader that Kalstein has for you, it meets the control requirements that achieves to obtain accurate test results, with the special use of quartz fiber optic system of the reader, which allows to decrease light to provide efficient readings throughout the microplate, offering a spectral value that guarantees the accuracy of DNA absorbance measurements.

Proper Use of Microplate Reader

To begin sample collection and storage, it is recommended that all sample calibrators and controls be processed in duplicate to calculate the results. This sample should be analyzed for 24 hours at temperatures of +4ยฐC quickly, to avoid freezing and thawing cycles. To do this, it recommends following the following procedures:

  • The user should thoroughly review all procedures.
  • Monitoring of all laboratory practices and safety standards should be considered.
  • Avoid mixing non-batch reagents.
  • Protect the current plates from any bright light or direct sunlight during practice.
  • Calibration is essential to avoid foaming.
  • Mix all reagents gently to avoid reading errors.
  • Testing is for professional use only.
  • In case only a positive or negative result is needed, it is not recommended to dilute the samples and process again when the requirements are otherwise met.
  • The samples should be diluted and processed to know the actual concentration if absorbance values are shown outside the calibration curve.

Kalstein Brand Microplate Reader

At Kalstein we are manufacturers of the best Microplate Readers on the market and at the best prices, they correspond to the YR series. It has the best technology to check the accuracy of your absorbance calculations with the use of traceable materials to ensure the final results. Provides linearity of R> 0.999, repeatability of < 0.3%, stability of <ยฑ0.001Abs/10min. In addition to a long-lasting high-light LED source, and the external computer that has ample storage capacity for 1,000 test items and 1,000,000 results.

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